Download e-book for iPad: A Guide to Methods in the Biomedical Sciences by Ronald B. Corley
By Ronald B. Corley
Hundreds of thousands of tools were built within the quite a few biomedical disciplines, and people coated during this publication characterize the fundamental, crucial and most generally used tools in different varied disciplines.
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For western blot analysis, antibodies are the most common probes. The antibodies are directly labeled with a radioactive molecule, such as so that its binding is detectable by autoradiography. Alternatively, it can be labeled with an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). The blot can then be treated with the appropriate substrate solution, which is converted into an insoluble material which identifies the location of the antibody bound to the protein of interest on the membrane.
DNA sequencing Introduction DNA sequencing is, as the name implies, a method that reveals the linear sequence of nucleic acids within a stretch of DNA. DNA is composed of four nucleotide bases: adenine (A), cytosine (C), guanine (G), and thymine (T). The base present at a particular position in a DNA sequence is identified by the presence of a product of a chemical or enzymatic reaction in a gel. The ability to sequence DNA was a major advance for a number of reasons. First, it allows for the prediction of a potential open reading frame (ORF) for genes within a genomic DNA sequence.
Restriction enzymes come from bacteria. They are a component of the “restriction-modification systems” of bacteria. These modification systems are thought to have evolved to help protect bacteria against foreign DNA, whether introduced by viral infection (by bacteriophages) or by DNA from other species of bacteria taken up by the host bacterium (see below). The fundamental work on restriction modification systems by Werner Arber, including the discovery of restriction enzymes, helped lead to the first insights into their sequence specificity by Kelly and Smith in 1970 (12) and to their use to characterize restriction fragments of viral DNA by Danna and Nathans in 1971 (11).
A Guide to Methods in the Biomedical Sciences by Ronald B. Corley